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Aims: The study aims to determine the antibiotic susceptibility pattern, incidence of β-lactamase and extended-spectrum β-lactamase (ESBL) production in Staphylococcus aureus isolated from surgical wounds. Study Design: A cross-sectional study designed to evaluate the incidence of antibiotic resistance and ESBL production in S. aureus recovered from surgical wound patients was conducted. The β-lactamase was assayed with the acidometric method while the ESBL was screened with the double disk approximation method. Conclusion: This study suggests a continuous screening and surveillance of MRSA among the patients in the hospital setting due to the high prevalence of these microorganisms in Osun state, Nigeria.